DSO may stand for:
- Distinguished Service Order
- Dallas Symphony Orchestra
- Darkstar Orchestra
- Deep sky object
- Defense Science Organization
- Defense Spectrum Office (US DoD)
- Detroit Symphony Orchestra
- Deutsche Stiftung Organtransplantation (dso.de)
- Deutsches Symphonie Orchester
- Digital storage oscilloscope
- Distributed Server Option
- District Staff Officer
- Dynamic shared object