The Guadalete River is a small stream located in the Spanish province of Cadiz, arising in the Grazalema Mountains at an elevation of about 1000 m, and running for 172 km into the Bay of Cadiz at El Puerto de Santa Maria, south of the city of Cadiz.

Along the way, it flows by some of "White Villages" of Andalusia, such as Arcos de la Frontera. The "Sierra Greenway", a 36 km bicycle path reclaimed from an abandoned railroad line, also passes along the river.

The river was once the frontier between Christian and Moorish Spain, receiving the sobriquet Rio de los Muertos (river of death).

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