Tress MacNeille (born June 20, 1957) is an American voice actor who is best known for providing various voices on the animated television show The Simpsons.

MacNeille has also provided voices on numerous other television shows and cartoons, including the voice of "Dot Warner" from the Animaniacs series, "Babs Bunny" from Tiny Toon Adventures, and "Gadget" from Chip 'n Dale Rescue Rangers. She has also recently done the voice of Daisy Duck in several Disney productions, and she frequently does dubbing work on English-language anime translations, as well.

MacNeille sang (as Lucille Ball) with Weird Al Yankovic on his song "Ricky", which was based on the I Love Lucy television show.